Want to extract breathing sound and heartbeat from silent audio file

Not directly. Nyquist plug-ins require Audacity.

You can however run Audacity from Bash, Python, or any other language that supports “named pipes”. This requires mod-script-pipe to be enabled in Audacity. Some info here: Scripting - Audacity Manual


See here: Manuals and reference material
and this section of the forum in general: Nyquist - Audacity Forum