I have been using Audacity for a couple of months on a Windows 10 Laptop using a second generation Focusrite 2i2 interface, with the PC having multiple playback options to send to another amplifier/sound system, the internal PC Speakers, and into the Focusrite out to a set of Monitors. I was in the middle of a recording session and the playback function within Audacity ceased to work. This lasted for a few hours then without me changing any of the settings it stopped being able to playback again during the session. The computer could tell that there was supposed to be audio coming out since it was showing the variance in the levels for the monitoring portion. I P then cycled through the Sounds settings on the PC and can clearly see that the Playback function with the Focusrite was functional since I could keep the same settings and have it play YouTube videos through the USB port connection of the Interface. The drivers for the interface are up to date as far as I know. The only thing I can really think to do would be to just install the program and reinstall.
If there is a glitch in the USB connection, Audacity may lose its connection. Transport > Rescan Audio Devices, should re-locate the device and you should be able to reestablish it as a recording or playback device with the Audio Setup button.
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