Transferred from the other topic:
That’s a lot of tracks.
Audio has to be buffered before it can play, which means copying a bit of audio from each track into memory (I don’t know which memory Windows uses for this, but it could be limited in size).
Are you able to merge some of the tracks so there are less tracks?
(If there is empty space in one track, an audio clip can be dragged vertically into the space using the Time Shift tool. Hold the Ctrl key down while you drag if you wish to retain the exact same time position.)