Try Windows cold shutdown. Shift - Shutdown. It will take longer than a regular restart or shutdown, but Windows resets and restarts a bunch more things when you do that.
Post back when you do that whether or not it worked. These “for no reason” failures are making everybody crazy.
By the way, we don’t make a 2.12. The current Audacity version is 2.1.3 and you can get it from here.
Windows 10, it started around October, I figured it was a clock error or sample mis match…but I have tried everything …Also the initial recording, the note “E” on a guitar is OK the first time around, however when the overdub record is recorded its playback is lower, about a B flat…I thought at first the pitch shift effect was on or processing the signal…because that’s kinda what it sounds like…I have disables all the effects, still no joy.
Updates all drivers, and using the latest Audacity release , and selected the box for resetting the preferences.